Coding
Part:BBa_K5374024:Design
Designed by: Langning Xu Group: iGEM24_TestDaily-I-Beijing (2024-09-24)
FTD-EGFP (Fibrinogen-like Domain). A fibrinogen-like domain involved in collagen and ECM remodeling,
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 163
Illegal PstI site found at 304
Illegal PstI site found at 629
Illegal PstI site found at 1096
Illegal PstI site found at 1237 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1438
Illegal PstI site found at 163
Illegal PstI site found at 304
Illegal PstI site found at 629
Illegal PstI site found at 1096
Illegal PstI site found at 1237 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 163
Illegal PstI site found at 304
Illegal PstI site found at 629
Illegal PstI site found at 1096
Illegal PstI site found at 1237 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 163
Illegal PstI site found at 304
Illegal PstI site found at 629
Illegal PstI site found at 1096
Illegal PstI site found at 1237 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2081
Design Notes
Sequence optimized for stable fusion with EGFP, retaining FTD’s collagen-binding function and ensuring reliable fluorescence for bioactivity studies.
Source
FTD domain from fibrinogen-related proteins, with EGFP from BBa_K1911005.